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KMID : 0861020180330040095
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Korea Journal of Herbology
2018 Volume.33 No. 4 p.95 ~ p.100
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Anti-Glyaction and Radical Scavenging Activities of Ligustri Fructus by Extraction Method
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Jeong Yun-Hee
Kim Seo-Yoon
Jeong Gyeong-Han
Kim Tae-Hoon
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Abstract
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Objectives : Ligustri Fructus has been used since ancient times as a medicinal usages in folk medicines against antitumor purpose. Many biological active constituents have been identified from this biomass such as several terpenoids and lignans. In current study, the properties of antioxidant and anti-diabetic complications using 1,1-diphenyl-2- picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS+) radicals scavenging, and advanced glycation end products (AGEs) inhibition assays were evaluated by different extraction methods of Ligustri Fructus.
Methods : In present continuing research for development of bioactive natural ingredients, antioxidant and AGEs formation inhibitory capacities of Ligustri Fructus extracts using different organic solvents were prepared and the biological potentials were investigated using in vitro bioassays. Antioxidant properties were evaluated employing radical scavenging assays using DPPH and ABTS+ radicals. In addition, the anti-diabetic complications effects of Ligustri Fructus extracts were tested via AGEs formation inhibitory assay. The total phenolic contents were determined using a spectrophotometric method.
Results : All the tested extracts exhibited dose-dependent radical scavenging and AGEs formation inhibitory activities. Among the tested samples, hot water extract of Ligustri Fructus was showed the most potent activity with IC50 value of 494.8±6.7 ㎍/㎖ against DPPH radical scavenging assay. Also, ABTS+ radical scavenging activity of hot water extract was higher than those of other extracts. In addition, AGEs formation inhibitory effects of each extacts and total phenolic contents were evaluated.
Conclusions : These results suggested that Ligustri Fructus can be considered as a new effective source of natural antioxidant and anti-diabetic complications resources. 1)
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KEYWORD
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Ligustri Fructus, total phenolic content, DPPH, ABTS+, advanced glycation end products (AGEs)
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